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The reproducibility of the EPR signal amplitudes between duplicate samples depended on sample packing density and the packing height within EPR tubes. Exposure of the parotid acinar cells to PP2, an inhibitor of Src and Src-related tyrosine kinases (36), also reduced the carbachol- and PMA-mediated increases in PKCδ tyrosine phosphorylation. Therefore every aspect of the experiments should be explained explicitly. Relevant notes will be placed close to where they are first mentioned. In this section, authors are expected to include any supplementary explanations that do not fit into the introduction and experimental part. These findings suggest that the use of rottlerin as an inhibitor of PKCs and PKCδ should be curtailed in all cells, regardless of the type of metabolism upon which they rely for energy production.

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