With this in mind, manufacturers are seeking to develop more streamlined sample-prep protocols that will facilitate timely sample loading. Sheared DNA was purified by binding to 0.6X volume of pre-washed AMPure XP beads (Beckman Coulter Inc.), as per PacBio protocol 000-710-821-DRAFT (five times in purified water, one time in EB, reconstituted in original supernatant) and eluted in EB to >60 ng/μl. Also, when the strand of DNA letters is translated to RNA, the T is exchanged for an U (uracil). This RNA is called messenger RNA (or mRNA). RNA, a molecule which resembles DNA, is however single-stranded.